Human Hepatocyte Nuclear Factor 1-alpha

Mfr.No.

E6690Hu

Sensitivity

7.13ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Hepatocyte nuclear factor 1-alpha;HNF1;HNF1A;HNF-1A;HNF1alpha;HNF-1-alpha;HNF4A;IDDM20;LFB1;Liver-specific transcription factor LF-B1;MODY3;TCF1;TCF-1;Transcription factor 1

Molecular Weight

67356

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6690Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	412.43	20.43	4.95
Intra-Assay	2	18	881.95	30.36	3.44
Intra-Assay	3	18	370.26	20.94	5.66

Background

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3' (PubMed:12453420, PubMed:10966642). Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Protocol

These standard curves of E6690Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.18	2.202	2.161
	2.225
800ng/L	1.352	1.413	1.372
	1.474
400ng/L	0.72	0.696	0.654
	0.672
200ng/L	0.421	0.382	0.34
	0.342
100ng/L	0.211	0.192	0.15
	0.173
0ng/L	0.038	0.042	0
	0.046

Gene ID	6927
UniProt ID	P20823

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115301	E6690Hu	48T	$320		Add to Cart
BHE12115301	E6690Hu	96T	$458		Add to Cart

Human Hepatocyte Nuclear Factor 1-alpha - HNF1A ELISA Kit

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