Human hepatocyte growth factor,HGF ELISA kit

Human hepatocyte growth factor,HGF ELISA kit

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Mfr.No.

CSB-E04573h

Alternative Names

hepatocyte growth factor (hepapoietin A; scatter factor), DFNB39, F-TCF, HGFB, HPTA, SF, OTTHUMP00000206710, fibroblast-derived tumor cytotoxic factor, hepatocyte growth factor, lung fibroblast-derived mitogen

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat BAX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	93-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BAX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	90
	Range %	87-99
1:8	Average %	93
	Range %	82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.091	1.995	2.043	1.844
2000	1.751	1.776	1.764	1.565
1000	1.368	1.432	1.400	1.201
500	0.985	0.972	0.979	0.780
250	0.535	0.544	0.540	0.341
125	0.348	0.352	0.350	0.151
62.5	0.244	0.237	0.241	0.042
0	0.201	0.197	0.199

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P14210
Antigen	hepatocyte growth factor (hepapoietin A; scatter factor)

Applications

ELISA

Research Area

HGF

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502702	CSB-E04573h	96T	$495	Add to Cart
BHE10502702	CSB-E04573h	5x96T	$2376	Add to Cart
BHE10502702	CSB-E04573h	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.