Human heparin cofactor II,HCIIELISA Kit

Mfr.No.

CSB-E09492h

Alternative Names

serpin peptidase inhibitor, clade D (heparin cofactor), member 1, D22S673, HC2, HCF2, HCII, HLS2, LS2, OTTHUMP00000198451, heparin cofactor II, leuserpin 2, serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1

Reactivity

Human

Detection Range

1.25 pg/mL-80 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	104	100-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	91
	Range %	85-96
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	95-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.692	2.600	2.646	2.497
1500	2.057	1.938	1.998	1.849
750	1.376	1.428	1.402	1.253
375	0.909	0.854	0.882	0.733
187.5	0.589	0.609	0.599	0.450
94	0.365	0.371	0.368	0.219
47	0.239	0.247	0.243	0.094
0	0.152	0.145	0.149	?

Species

Homo sapiens

Sensitivity

0.312 pg/mL

Limit Of Detection

450 nm

UniProt ID	P05546
Antigen	serpin peptidase inhibitor, clade D (heparin cofactor), member 1

Applications

ELISA

Research Area

SERPIND1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502677	CSB-E09492h	96T	$790	Add to Cart
BHE10502677	CSB-E09492h	5x96T	$3792	Add to Cart
BHE10502677	CSB-E09492h	10x96T	$7505	Add to Cart

Human heparin cofactor II,HCIIELISA Kit

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