Human heparin-binding epidermal growth factor-like growth factor,HB-EGF ELISA Kit

Human heparin-binding epidermal growth factor-like growth factor,HB-EGF ELISA Kit

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Mfr.No.

CSB-E09716h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

heparin-binding EGF-like growth factor, DTR, DTS, DTSF, HEGFL, diphtheria toxin receptor (heparin-binding EGF-like growth factor), diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor), heparin-binding epide

Recovery

The recovery of mouse sTLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-95
EDTA plasma (n=4)	102	97-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	95
	Range %	88-98
1:10	Average %	100
	Range %	96-103
1:20	Average %	94
	Range %	88-97
1:40	Average %	99
	Range %	95-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.758	2.713	2.736	2.619
5	2.012	2.087	2.050	1.933
2.5	1.204	1.178	1.191	1.074
1.25	0.663	0.652	0.658	0.541
0.625	0.378	0.366	0.372	0.255
0.312	0.275	0.281	0.278	0.161
0.156	0.194	0.187	0.191	0.074
0	0.116	0.118	0.117

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q99075

Applications	ELISA
Research Area	HBEGF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502676	CSB-E09716h	96T	$595	Add to Cart
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BHE10502676	CSB-E09716h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.