Human Heparan Sulfate Glucosamine 3-O-sulfotransferase 4

Mfr.No.

E5387Hu

Sensitivity

7.26ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

3-OST-4;h3-OST-4;Heparan sulfate 3-O-sulfotransferase 4;Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4;Heparan sulfate glucosamine 3-O-sulfotransferase 4;HS3ST4

Molecular Weight

49799

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5387Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1062.5	36.64	3.4
Intra-Assay	2	18	652.1	35.81	5.5
Intra-Assay	3	18	248.8	14.89	6

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate (By similarity).

Protocol

These standard curves of E5387Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.417	2.357	2.282
	2.297
800ng/L	1.312	1.31	1.236
	1.309
400ng/L	0.743	0.782	0.707
	0.82
200ng/L	0.466	0.464	0.389
	0.462
100ng/L	0.247	0.254	0.179
	0.262
0ng/L	0.076	0.075	0
	0.074

Gene ID	9951
UniProt ID	Q9Y661

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106750	E5387Hu	48T	$320		Add to Cart
BHE12106750	E5387Hu	96T	$458		Add to Cart

Human Heparan Sulfate Glucosamine 3-O-sulfotransferase 4 - HS3ST4 ELISA Kit

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