Human Hemoglobin (Hb) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16994h

Alternative Names

Hemoglobin (Hb)

Reactivity

Human

Detection Range

0.244 ng/mL-1000 ng/mL

Sample Type

Lysate for rbc, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ELA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	108	98-110
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ELA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-95
1:2	Average %	85
	Range %	81-89
1:4	Average %	95
	Range %	91-99
1:8	Average %	97
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.244 ng/mL

Limit Of Detection

450 nm

Antigen	Hemoglobin (HB)

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Extravascular red blood cells and hemoglobin promote tumor growth and therapeutic resistance as endogenous danger signals. Yin T.et al,J Immunol.,194(1):429-37.

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