Human Hemoglobin (Hb) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16994h

Sensitivity

0.244 ng/mL

Detection Range

0.244 ng/mL-1000 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Hemoglobin (Hb)

Recovery

The recovery of human ELA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	108	98-110
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ELA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-95
1:2	Average %	85
	Range %	81-89
1:4	Average %	95
	Range %	91-99
1:8	Average %	97
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Hb

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Extravascular red blood cells and hemoglobin promote tumor growth and therapeutic resistance as endogenous danger signals. Yin T.et al,J Immunol.,194(1):429-37.

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