Human Hemogen - HEMGN ELISA Kit

Mfr.No.

E2740Hu

Sensitivity

0.096ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Hemogen (also known as HEMGN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CT155;EDAG;EDAG-1;Erythroid differentiation-associated gene protein;HEMGN;Hemogen;Hemopoietic gene protein;NDR;Negative differentiation regulator protein;

Molecular Weight

55341

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2740Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.74	0.24	5.17
Intra-Assay	2	18	13.98	0.38	2.73
Intra-Assay	3	18	7.85	0.49	6.28

Background

Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).

Protocol

These standard curves of E2740Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.322	2.319	2.265
	2.317
20ng/ml	1.715	1.721	1.666
	1.726
10ng/ml	1.089	1.167	1.113
	1.246
5ng/ml	0.695	0.671	0.617
	0.647
2.5ng/ml	0.44	0.436	0.381
	0.432
0ng/ml	0.045	0.055	0
	0.064

Gene ID	55363
UniProt ID	Q9BXL5

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104277	E2740Hu	48T	$320		Add to Cart
BHE12104277	E2740Hu	96T	$458		Add to Cart

Human Hemogen - HEMGN ELISA Kit

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