Human Heat Shock Protein glycoprotein 96,HSP gp96 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08551h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Heat Shock Protein glycoprotein 96,HSP gp96

Recovery

The recovery of human CYP3A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-103
EDTA plasma (n=4)	98	95-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP3A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-99
1:2	Average %	96
	Range %	90-102
1:4	Average %	97
	Range %	89-105
1:5	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P14625

Applications	ELISA
Research Area	HSP gp96

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502653	CSB-E08551h	10x96T	$7505	Add to Cart