Human Heat Shock Protein Beta 7

Mfr.No.

E5380Hu

Sensitivity

0.098ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cardiovascular heat shock protein;cvHSP;Heat shock protein beta-7;HspB7

Molecular Weight

18611

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5380Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.42	0.4	6.25
Intra-Assay	2	18	15.6	0.57	3.62
Intra-Assay	3	18	9.45	0.53	5.6

Background

Heat Shock Protein Family B (small) member 7 (HSPB7) in humans is a protein encoded by a gene of the same name with four exons that is located on chromosome 1p36.13. HSPB7 contains 170 amino acids and has a molecular weight of 18,611Da. HSPB7 is a member of human small heat shock protein (HSPB) family, which contains eleven family members of chaperone proteins.(7) HSPB7 and its gene pair SRARP are located 5 kb apart on the opposite strands of chromosome 1p36.13.

Protocol

These standard curves of E5380Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.163	2.182	2.134
	2.2
16ng/ml	1.304	1.297	1.25
	1.29
8ng/ml	0.829	0.856	0.809
	0.884
4ng/ml	0.503	0.496	0.448
	0.489
2ng/ml	0.28	0.287	0.239
	0.294
0ng/ml	0.043	0.047	0
	0.052

Gene ID	27129
UniProt ID	Q9UBY9

Research Area	Signal Transduction; Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106743	E5380Hu	48T	$320		Add to Cart
BHE12106743	E5380Hu	96T	$458		Add to Cart

Human Heat Shock Protein Beta 7 - HSPB7 ELISA Kit

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