Human heat shock protein 90alpha (HSP-90alpha) ELISA kit

Human heat shock protein 90alpha (HSP-90alpha) ELISA kit

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Mfr.No.

CSB-E13462h

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

heat shock protein 90alpha (HSP-90alpha), FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, a, heat shock protein 90alpha (HSP-90alpha), heat shock protein 90 alpha (HSP-90 alpha)

Recovery

The recovery of rat CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-102
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-97
1:2	Average %	95
	Range %	91-99
1:4	Average %	98
	Range %	96-101
1:8	Average %	99
	Range %	96-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.625	2.529	2.577	2.413
1000	2.062	1.956	2.009	1.845
500	1.384	1.366	1.375	1.211
250	0.871	0.881	0.876	0.712
125	0.551	0.539	0.545	0.381
62.5	0.342	0.351	0.347	0.183
31.25	0.249	0.255	0.252	0.088
0	0.164	0.163	0.164	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P07900

Applications	ELISA
Research Area	HSP90AA1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502645	CSB-E13462h	96T	$790	Add to Cart
BHE10502645	CSB-E13462h	5x96T	$3792	Add to Cart
BHE10502645	CSB-E13462h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.