Human Heat Shock Protein 75 kDa - Mitochondrial

Mfr.No.

E5375Hu

Alternative Names

Heat shock protein 75 kDa, mitochondrial;HSP 75;HSP75;HSP90L;TNFR-associated protein 1;TRAP1;TRAP-1;Tumor necrosis factor type 1 receptor-associated protein

Reactivity

Human

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

80110

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.14ng/mL

Background

Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.

Gene ID	10131
UniProt ID	Q12931
Antigen	TRAP1

Research Area

Others

Protocol

These standard curves of E5375Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.269	2.12	2.087
	1.97
24ng/mL	1.536	1.518	1.485
	1.5
12ng/mL	0.728	0.687	0.655
	0.647
6ng/mL	0.348	0.377	0.344
	0.405
3ng/mL	0.181	0.166	0.134
	0.152
0ng/mL	0.028	0.032	0
	0.037

Precision

We measured random samples of E5375Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.52	0.46	4.8
Intra-Assay	2	18	13.95	0.62	4.45
Intra-Assay	3	18	27.67	1.04	3.76

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106738	E5375Hu	48T	$320		Add to Cart
BHE12106738	E5375Hu	96T	$458		Add to Cart

Human Heat Shock Protein 75 kDa - Mitochondrial - HSP75 ELISA Kit

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