Human Heat Shock Protein 72(HSP-72) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14916h

Alternative Names

Heat Shock Protein 72(HSP-72)

Reactivity

Human

Detection Range

3.12 ng/mL-200 ng/mL

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat CYP7A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	96	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CYP7A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-92
1:2	Average %	96
	Range %	92-99
1:4	Average %	98
	Range %	93-103
1:8	Average %	95
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.78 ng/mL

Limit Of Detection

450 nm

UniProt ID	P0DMV8
Antigen	Heat Shock Protein 72(HSP-72)

Applications

ELISA

Research Area

HSP72

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Heat acclimation has a protective effect on the central but not peripheral nervous system Racinais S.et al,J Appl Physiol ,Jul 6

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