Human Heat Shock Protein 70,HSP-70 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08297h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

heat shock 70kDa protein 1B, DAAP-21F2.7, FLJ54328, HSP70-1B, HSP70-2, HSPA1A, heat shock 70kD protein 1B

Recovery

The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	9-103
EDTA plasma (n=4)	93	89-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	93
	Range %	89-97
1:4	Average %	92
	Range %	84-96
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P0DMV9

Applications	ELISA
Research Area	HSPA1B

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502642	CSB-E08297h	10x96T	$7505	Add to Cart