Human Heat Shock 70kDa Protein 4

Mfr.No.

E3016Hu

Sensitivity

0.018ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

APG-2;Heat shock 70 kDa protein 4;Heat shock 70-related protein APG-2;HEL-S-5a;hsp70;hsp70RY;HSPA4;HSPH2;RY;

Molecular Weight

94331

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3016Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.75	0.27	4.73
Intra-Assay	2	18	0.77	0.05	6.27
Intra-Assay	3	18	2.74	0.22	8.2

Background

Heat shock 70 kDa protein 4 is a protein that in humans is encoded by the HSPA4 gene. The protein encoded by this gene was originally suggested to be a member of the heat shock protein 70 family.) However it is now known that human HSPA4 is an equivalent to mouse the Apg-2 protein and is a member of the Hsp110 family.

Protocol

These standard curves of E3016Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.164	2.207	2.167
	2.249
6ng/ml	1.738	1.618	1.578
	1.497
3ng/ml	0.979	1.036	0.997
	1.093
1.5ng/ml	0.633	0.635	0.595
	0.637
0.75ng/ml	0.421	0.442	0.402
	0.463
0ng/ml	0.051	0.04	0
	0.028

Gene ID	3308
UniProt ID	P34932

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104553	E3016Hu	48T	$320		Add to Cart
BHE12104553	E3016Hu	96T	$458		Add to Cart

Human Heat Shock 70kDa Protein 4 - HSPA4 ELISA Kit

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