Human heart fatty acid binding protein,h-FABP ELISA Kit

Mfr.No.

CSB-E09185h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor), FABP11, H-FABP, MDGI, O-FABP, Fatty acid-binding protein 3, muscle, fatty acid binding protein 11, fatty acid binding protein 3, mammary-derived growth inhibitor

Recovery

The recovery of mouse GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-92
EDTA plasma (n=4)	94	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	104
	Range %	97-108
1:4	Average %	92
	Range %	87-98
1:8	Average %	91
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.421	2.536	2.479	2.288
750	1.905	2.011	1.958	1.767
375	1.328	1.413	1.371	1.180
187.5	0.821	0.834	0.828	0.637
94	0.514	0.527	0.521	0.330
47	0.370	0.387	0.379	0.188
23.5	0.311	0.315	0.313	0.122
0	0.190	0.192	0.191	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P05413

Applications	ELISA
Research Area	FABP3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502633	CSB-E09185h	96T	$790	Add to Cart
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BHE10502633	CSB-E09185h	10x96T	$7505	Add to Cart

Human heart fatty acid binding protein,h-FABP ELISA Kit

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