Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of HDC and the recovery rates were calculated by comparing the measured value to the expected amount of HDC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
92
EDTA plasma(n=5)
90-103
98
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HDC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-103%
87-99%
85-101%
EDTA plasma(n=5)
85-101%
83-101%
82-97%
heparin plasma(n=5)
80-94%
93-98%
80-94%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
P19113
Applications
ELISA
Applications Notes
HDC ELISA Kit allows for the in vitro quantitative determination of HDC concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HDC (Histidine decarboxylase) ELISA Kit