Human HCV (Hepatitis C Virus) ELISA Kit

Mfr.No.

EH4963

Description

This kit is based on Double antibody-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti HCV antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HCV bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HCV in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Alternative Names

HCV ELISA Kit, Hepatitis C Virus ELISA Kit

Reactivity

Human

Detection Range

Qualitative

Assay Type

Qualitative

Assay Duration

3 hours

Sensitivity

Qualitative

Specificity

Specifically binds with HCV , no obvious cross reaction with other analogues.

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

For Research Use Only

Usage Notes	Step 1: Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C. Washing: Wash the plate twice without immersion. Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C. Washing: Wash the plate five times without immersion. Step 3: Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.) Step 4: Add 50ul stop solution. Read at 450nm immediately and calculate.

Applications	ELISA
Applications Notes	HCV ELISA Kit allows for the in vitro quantitative determination of HCV concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808228	EH4963	48T	$280		Add to Cart
BHE10808228	EH4963	96T	$400		Add to Cart

Human HCV (Hepatitis C Virus) ELISA Kit

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