Matrices listed below were spiked with certain level of sAb and the recovery rates were calculated by comparing the measured value to the expected amount of sAb in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
95
EDTA plasma(n=5)
90-101
96
heparin plasma(n=5)
91-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sAb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.