Matrices listed below were spiked with certain level of HACE1 and the recovery rates were calculated by comparing the measured value to the expected amount of HACE1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
93
EDTA plasma(n=5)
87-104
95
heparin plasma(n=5)
86-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HACE1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HACE1/HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1/HACE1/KIAA1320/6.3.2.-
Notes
For Research Use Only
UniProt ID
Q8IYU2
Antigen
HACE1
Applications
ELISA
Applications Notes
HACE1 ELISA Kit allows for the in vitro quantitative determination of HACE1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human HACE1(E3 ubiquitin-protein ligase HACE1) ELISA Kit