Human H-Cadherin - H-CAD ELISA Kit

Mfr.No.

E0245Hu

Description

This sandwich kit is for the accurate quantitative detection of human H-Cadherin (also known as H-Cad/CDH13) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cadherin-13;CDH 13;CDH13;H-CAD;H-cadherin;Heart cadherin;P105;T-cad;T-cadherin;Truncated cadherin

Reactivity

Human

Detection Range

0.1-40.ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

78287

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.051ng/ml

Background

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.

Gene ID	1012
UniProt ID	P55290
Antigen	CDH13

Research Area

Cancer

Protocol

These standard curves of E0245Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.415	2.254	2.218
	2.094
12ng/ml	1.301	1.241	1.204
	1.181
6ng/ml	0.767	0.67	0.633
	0.573
3ng/ml	0.413	0.368	0.332
	0.324
1.5ng/ml	0.214	0.204	0.167
	0.194
0ng/ml	0.035	0.037	0
	0.039

Precision

We measured random samples of E0245Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.1	0.39	2.7
Intra-Assay	2	18	8.4	0.45	5.3
Intra-Assay	3	18	6.7	0.43	6.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101960	E0245Hu	96T	$458		Add to Cart
BHE12101960	E0245Hu	48T	$320		Add to Cart

Human H-Cadherin - H-CAD ELISA Kit

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