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Home / ELISA Kit

Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit

SKU : BHE13703605

NAF1 (Nuclear Assembly Factor 1 Ribonucleoprotein) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and RNA binding. RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.

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Mfr.No.	KTE61390
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAF1.
Alternative Names	NAF1; Nuclear assembly factor 1 homolog
Detection Method	Colorimetric
Kit Components	Human H/ACA ribonucleoprotein complex non-core subunit NAF1 microplate Human H/ACA ribonucleoprotein complex non-core subunit NAF1 standard Human H/ACA ribonucleoprotein complex non-core subunit NAF1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAF1. No significant cross-reactivity or interference between Human NAF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	NAF1 (Nuclear Assembly Factor 1 Ribonucleoprotein) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and RNA binding. RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
Notes	This Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAF1 in samples. An antibody specific for NAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	92345

Applications	ELISA
Applications Notes	This Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAF1 in samples. An antibody specific for NAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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