Matrices listed below were spiked with certain level of GZMK and the recovery rates were calculated by comparing the measured value to the expected amount of GZMK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
93
EDTA plasma(n=5)
86-103
97
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GZMK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GZMK ELISA Kit allows for the in vitro quantitative determination of GZMK concentrations in serum, plasma, tissue homogenates and other biological fluids.