Human guanylate cyclase activator 2B (GUCA2B) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL010048HU

Alternative Names

guanylate cyclase activator 2B, GCAP-II, UGN, guanylate cyclase activator 2B, uroguanylin

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Serum, Plasma, Urine, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TLR4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-103
EDTA plasma (n=4)	97	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TLR4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	85
	Range %	80-93
1:10	Average %	95
	Range %	89-100
1:20	Average %	97
	Range %	93-104
1:40	Average %	95
	Range %	91-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q16661
Antigen	guanylate cyclase activator 2B (uroguanylin)

Applications

ELISA

Research Area

GUCA2B

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502624	CSB-EL010048HU	96T	$595	Add to Cart
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