Human Gtp Cyclohydrolase 1

Mfr.No.

E5366Hu

Sensitivity

7.47ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GCH 1;GCH1;GTP cyclohydrolase 1;GTP cyclohydrolase I;GTP-CH-I

Molecular Weight

27903

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5366Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1295.7	37.97	2.9
Intra-Assay	2	18	985.6	40.17	4.1
Intra-Assay	3	18	401.8	20.32	5.1

Background

Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.

Protocol

These standard curves of E5366Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.928	1.972	1.924
	2.016
800ng/L	0.898	0.907	0.858
	0.915
400ng/L	0.514	0.544	0.496
	0.574
200ng/L	0.166	0.222	0.173
	0.278
100ng/L	0.101	0.082	0.034
	0.064
0ng/L	0.04	0.049	0
	0.057

Gene ID	2643
UniProt ID	P30793

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106729	E5366Hu	48T	$320		Add to Cart
BHE12106729	E5366Hu	96T	$458		Add to Cart

Human Gtp Cyclohydrolase 1 - GCH1 ELISA Kit

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