JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / ELISA Kit

Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit

SKU : BHE13702853

The deduced 227-amino acid protein has a calculated molecular mass of 25.6 kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1). It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecular mass of 28 kD in all tissues examined. Subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction.Human embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA.

Continuing the saving: $50 Off All ELISA Kits Offer Extended!

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Abbkine Scientific Co., Ltd.

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	KTE60637
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit employs a two-site sandwich ELISA to quantitate AK3.
Alternative Names	AK3; RP11-6J24.4; AK3L1; AK6; AKL3L; AKL3L1; FIX; adenylate kinase 3 alpha like; adenylate kinase 3 like 1; adenylate kinase 6; adenylate kinase 3 like 1
Detection Method	Colorimetric
Kit Components	Human GTP:AMP phosphotransferase, mitochondrial microplate Human GTP:AMP phosphotransferase, mitochondrial standard Human GTP:AMP phosphotransferase, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AK3. No significant cross-reactivity or interference between Human AK3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced 227-amino acid protein has a calculated molecular mass of 25.6 kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1). It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecular mass of 28 kD in all tissues examined. Subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction.Human embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA.
Notes	This Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit employs a two-site sandwich ELISA to quantitate AK3 in samples. An antibody specific for AK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	50808

Applications	ELISA
Applications Notes	This Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit employs a two-site sandwich ELISA to quantitate AK3 in samples. An antibody specific for AK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHE13702853	KTE60637	48 T	$339		Add to Cart
BHE13702853	KTE60637	96 T	$589		Add to Cart
BHE13702853	KTE60637	96 Tx5	$2629		Add to Cart
BHE13702853	KTE60637	96 Tx50	$24569		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.