Matrices listed below were spiked with certain level of GSPT1 and the recovery rates were calculated by comparing the measured value to the expected amount of GSPT1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
95
EDTA plasma(n=5)
93-104
99
heparin plasma(n=5)
88-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GSPT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
86-103%
85-104%
EDTA plasma(n=5)
83-97%
82-99%
83-100%
heparin plasma(n=5)
80-98%
82-99%
82-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
GSPT1 ELISA Kit allows for the in vitro quantitative determination of GSPT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GSPT1 (Eukaryotic peptide chain release factor GTP-binding subunit ERF3A) ELISA Kit