Human Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) ELISA kit

Human Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) ELISA kit

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Mfr.No.

CSB-EL009161HU

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

growth arrest and DNA-damage-inducible, alpha, RP5-975D15.1, DDIT1, GADD45, DNA damage-inducible transcript 1, DNA damage-inducible transcript-1, DNA-damage-inducible transcript 1, growth arrest and DNA-damage-inducible 45 alpha

Recovery

The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	90
	Range %	87-93
1:200	Average %	94
	Range %	90-98
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.569	2.547	2.558	2.429
50	2.236	2.213	2.225	2.096
25	1.868	1.829	1.849	1.720
12.5	1.367	1.387	1.377	1.248
6.25	0.840	0.889	0.865	0.736
3.13	0.377	0.387	0.382	0.253
1.56	0.247	0.259	0.253	0.124
0	0.128	0.130	0.129	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P24522

Applications	ELISA
Research Area	GADD45A

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502599	CSB-EL009161HU	96T	$695	Add to Cart
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BHE10502599	CSB-EL009161HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.