Human Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) ELISA kit

Human Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) ELISA kit

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Mfr.No.

CSB-EL009161HU

Alternative Names

growth arrest and DNA-damage-inducible, alpha, RP5-975D15.1, DDIT1, GADD45, DNA damage-inducible transcript 1, DNA damage-inducible transcript-1, DNA-damage-inducible transcript 1, growth arrest and DNA-damage-inducible 45 alpha

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	90
	Range %	87-93
1:200	Average %	94
	Range %	90-98
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.569	2.547	2.558	2.429
50	2.236	2.213	2.225	2.096
25	1.868	1.829	1.849	1.720
12.5	1.367	1.387	1.377	1.248
6.25	0.840	0.889	0.865	0.736
3.13	0.377	0.387	0.382	0.253
1.56	0.247	0.259	0.253	0.124
0	0.128	0.130	0.129	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P24522
Antigen	growth arrest and DNA-damage-inducible, alpha

Applications

ELISA

Research Area

GADD45A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502599	CSB-EL009161HU	96T	$695	Add to Cart
BHE10502599	CSB-EL009161HU	5x96T	$3336	Add to Cart
BHE10502599	CSB-EL009161HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.