Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of GRM5 and the recovery rates were calculated by comparing the measured value to the expected amount of GRM5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-101
96
EDTA plasma(n=5)
87-104
95
heparin plasma(n=5)
87-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GRM5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
92-102%
90-104%
EDTA plasma(n=5)
82-98%
82-96%
87-99%
heparin plasma(n=5)
81-97%
80-95%
84-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P41594
Applications
ELISA
Applications Notes
GRM5 ELISA Kit allows for the in vitro quantitative determination of GRM5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GRM5 (Metabotropic glutamate receptor 5) ELISA Kit