Human Gremlin-2(GREM2) ELISA kit

Human Gremlin-2(GREM2) ELISA kit

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Mfr.No.

CSB-EL009893HU

Sensitivity

6.25 pg/mL

Detection Range

6.25 pg/mL-2500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis), CKTSF1B2, DAND3, PRDC, cysteine knot superfamily 1, BMP antagonist 2, gremlin 2, protein related to DAN and cerberus

Recovery

The recovery of human MFI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MFI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	103
	Range %	99-108
1:4	Average %	90
	Range %	85-94
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.486	2.315	2.401	2.296
5	2.034	1.974	2.004	1.899
2.5	1.506	1.414	1.460	1.355
1.25	1.027	0.981	1.004	0.899
0.625	0.631	0.663	0.647	0.542
0.312	0.348	0.325	0.337	0.232
0.156	0.241	0.257	0.249	0.144
0	0.107	0.103	0.105

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9H772

Applications	ELISA
Research Area	GREM2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502595	CSB-EL009893HU	96T	$695	Add to Cart
BHE10502595	CSB-EL009893HU	5x96T	$3336	Add to Cart
BHE10502595	CSB-EL009893HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.