Human GREM2/Prdc ELISA Kit PicoKine®

Mfr.No.

EK1533

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.090	0.171	0.211	0.345	0.532	0.890	1.558	2.342

Description

Human GREM2/Prdc ELISA Kit PicoKine® (96 Tests). Quantitate Human GREM2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Gremlin-2;Cysteine knot superfamily 1, BMP antagonist 2;DAN domain family member 3;Protein related to DAN and cerberus;GREM2;CKTSF1B2, DAND3, PRDC;

Molecular Weight

19320 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R22-Q168

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human GREM2

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	142	522	1488	140	556	1359
Standard deviation	10.08	39.15	113.08	12.32	45.03	107.36
CV (%)	7.1%	7.5%	7.6%	8.8%	8.1%	7.9%

Protein Function

Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells (By similarity). .

Background

GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	142	139	149	126	139	8.33	5.9%
Sample 2	522	566	575	538	550	21.26	3.8%
Sample 3	1488	1697	1588	1757	1632	103.1	6.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	64388
Gene Name	GREM2
UniProt ID	Q9H772
Protein Name	Gremlin-2

Applications	ELISA
Research Area	Cytokines,Cytoskeleton/ECM,Embryonic Stem Cells,Extracellular Matrix,Signal Transduction,Stem Cells,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001114	EK1533	96 wells/kit	$499		Add to Cart

Human GREM2/Prdc ELISA Kit PicoKine®

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