Human Grb2-associated-binding Protein 2

Mfr.No.

E5360Hu

Alternative Names

GAB 2;GAB2;GRB2-associated binder 2;GRB2-associated-binding protein 2;Growth factor receptor bound protein 2-associated protein 2;pp100

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

74458

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.29ng/L

Background

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

Gene ID	9846
UniProt ID	Q9UQC2
Antigen	GAB2

Research Area

Others

Protocol

These standard curves of E5360Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.451	2.442	2.398
	2.433
800ng/L	1.36	1.393	1.348
	1.425
400ng/L	0.767	0.799	0.755
	0.832
200ng/L	0.554	0.553	0.509
	0.552
100ng/L	0.244	0.259	0.214
	0.273
0ng/L	0.048	0.044	0
	0.041

Precision

We measured random samples of E5360Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1034	32.31	3.1
Intra-Assay	2	18	394.7	18.81	4.8
Intra-Assay	3	18	220	15.41	7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106723	E5360Hu	48T	$320		Add to Cart
BHE12106723	E5360Hu	96T	$458		Add to Cart

Human Grb2-associated-binding Protein 2 - GAB2 ELISA Kit

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