Human granzyme B (GZMB) ELISA Kit

Mfr.No.

CSB-E08718h

Alternative Names

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, T-cell serine protease 1-3E, cathepsin G-like 1, cytotoxic serine protease B, fragmentin 2, granzyme B

Reactivity

Human

Detection Range

1.56 pg/mL-100 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of dog SELP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	102	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog SELP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	104
	Range %	100-108
1:4	Average %	86
	Range %	83-90
1:8	Average %	105
	Range %	100-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.584	2.468	2.526	2.402
400	1.533	1.534	1.534	1.410
200	0.985	0.990	0.988	0.864
100	0.571	0.581	0.576	0.452
50	0.365	0.346	0.356	0.232
25	0.262	0.271	0.267	0.143
12.5	0.194	0.196	0.195	0.071
0	0.123	0.125	0.124	?

Species

Homo sapiens

Sensitivity

0.39 pg/mL

Limit Of Detection

450 nm

UniProt ID	P10144
Antigen	granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

Applications

ELISA

Research Area

GZMB

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502592	CSB-E08718h	96T	$468	Add to Cart
BHE10502592	CSB-E08718h	5x96T	$2246.40	Add to Cart
BHE10502592	CSB-E08718h	10x96T	$4446	Add to Cart

Human granzyme B (GZMB) ELISA Kit

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