Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Mfr.No.

CSB-E04563h

Sensitivity

1.95 pg/ml

Detection Range

7.8 pg/ml -500 pg/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

colony stimulating factor 3 (granulocyte), G-CSF, GCSF, MGC45931, colony stimulating factor 3, filgrastim, granulocyte colony stimulating factor, lenograstim, pluripoietin

Recovery

The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	102	96-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	96
	Range %	89-100
1:200	Average %	90
	Range %	85-95
1:400	Average %	100
	Range %	94-104
1:800	Average %	105
	Range %	97-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1000	0.122	0.120	0.121
500	0.166	0.158	0.162
250	0.232	0.241	0.237
125	0.337	0.344	0.341
62.5	0.528	0.510	0.519
31.25	0.941	0.955	0.948
15.625	1.471	1.522	1.497
0	2.326	2.419	2.373

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P09919

Applications	ELISA
Research Area	CSF3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502584	CSB-E04563h	96T	$468	Add to Cart
BHE10502584	CSB-E04563h	5x96T	$2246.40	Add to Cart
BHE10502584	CSB-E04563h	10x96T	$4446	Add to Cart

Human Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

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