Human Granulocyte Chemotactic Protein-2

Mfr.No.

E0172Hu

Sensitivity

0.022ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Granulocyte Chemotactic Protein-2 (also known as GCP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Chemokine alpha 3;CKA-3;C-X-C motif chemokine 6;CXCL 6;CXCL6;GCP-2;Granulocyte chemotactic protein 2;Small-inducible cytokine B6;Small-inducible cytokine B6, N-processed variant 1;Small-inducible cytokine B6, N-processed variant 2;Small-inducible cytokine B6, N-processed variant 3

Molecular Weight

11897

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0172Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.2	0.19	4.4
Intra-Assay	2	18	0.7	0.04	5.4
Intra-Assay	3	18	1.9	0.09	4.8

Background

Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).

Protocol

These standard curves of E0172Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.298	2.262	2.204
	2.225
3.2ng/ml	1.459	1.47	1.412
	1.481
1.6ng/ml	0.862	0.809	0.751
	0.756
0.8ng/ml	0.515	0.486	0.428
	0.456
0.4ng/ml	0.291	0.294	0.236
	0.297
0ng/ml	0.067	0.058	0
	0.049

Gene ID	6372
UniProt ID	P80162

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101907	E0172Hu	96T	$458		Add to Cart
BHE12101907	E0172Hu	48T	$320		Add to Cart

Human Granulocyte Chemotactic Protein-2 - GCP-2 ELISA Kit

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