Human Granulins(GRN) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL009939HU

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

granulin, GEP, GP88, PCDGF, PEPI, PGRN, PC cell-derived growth factor, acrogranin, granulin-epithelin, proepithelin, progranulin

Recovery

The recovery of human MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	101	96-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	100
	Range %	95-115
1:200	Average %	97
	Range %	91-111
1:400	Average %	97
	Range %	92-106
1:800	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Cell culture supeatants, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P28799
Tissue Specificity	In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

Applications	ELISA
Research Area	GRN

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502581	CSB-EL009939HU	96T	$695	Add to Cart
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BHE10502581	CSB-EL009939HU	10x96T	$6602.50	Add to Cart