Human Granulins(GRN) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL009939HU

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

granulin, GEP, GP88, PCDGF, PEPI, PGRN, PC cell-derived growth factor, acrogranin, granulin-epithelin, proepithelin, progranulin

Recovery

The recovery of human MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	101	96-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	100
	Range %	95-115
1:200	Average %	97
	Range %	91-111
1:400	Average %	97
	Range %	92-106
1:800	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	P28799
Antigen	Granulin

Applications

ELISA

Research Area

GRN

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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