Matrices listed below were spiked with certain level of GPX4 and the recovery rates were calculated by comparing the measured value to the expected amount of GPX4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
96
EDTA plasma(n=5)
89-105
98
heparin plasma(n=5)
93-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
97-103%
94-99%
94-100%
EDTA plasma(n=5)
83-99%
82-101%
85-93%
heparin plasma(n=5)
81-100%
82-98%
92-99%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
P36969
Applications
ELISA
Applications Notes
GPX4 ELISA Kit allows for the in vitro quantitative determination of GPX4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GPX4 (Phospholipid hydroperoxide glutathione peroxidase)ELISA Kit