Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of GPR65 and the recovery rates were calculated by comparing the measured value to the expected amount of GPR65 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
93
EDTA plasma(n=5)
90-103
97
heparin plasma(n=5)
91-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPR65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-100%
89-102%
96-104%
EDTA plasma(n=5)
82-99%
88-97%
85-94%
heparin plasma(n=5)
85-99%
85-100%
87-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q8IYL9
Applications
ELISA
Applications Notes
GPR65 ELISA Kit allows for the in vitro quantitative determination of GPR65 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GPR65 (Psychosine receptor) ELISA Kit