Matrices listed below were spiked with certain level of GPM6A and the recovery rates were calculated by comparing the measured value to the expected amount of GPM6A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-104
92
EDTA plasma(n=5)
87-105
97
heparin plasma(n=5)
85-96
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPM6A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-103%
85-97%
85-104%
EDTA plasma(n=5)
83-96%
86-94%
84-100%
heparin plasma(n=5)
85-100%
85-100%
82-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P51674
Applications
ELISA
Applications Notes
GPM6A ELISA Kit allows for the in vitro quantitative determination of GPM6A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human GPM6A(Neuronal membrane glycoprotein M6-a) ELISA Kit