Matrices listed below were spiked with certain level of GPC4 and the recovery rates were calculated by comparing the measured value to the expected amount of GPC4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
99
EDTA plasma(n=5)
87-105
94
heparin plasma(n=5)
85-101
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPC4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GPC4 ELISA Kit allows for the in vitro quantitative determination of GPC4 concentrations in serum, plasma, tissue homogenates and other biological fluids.