Matrices listed below were spiked with certain level of GPC-6 and the recovery rates were calculated by comparing the measured value to the expected amount of GPC-6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
94
EDTA plasma(n=5)
80-96
91
heparin plasma(n=5)
88-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPC-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
82-103%
80-89%
86-100%
EDTA plasma(n=5)
90-101%
90-100%
90-98%
heparin plasma(n=5)
93-100%
91-94%
92-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q9Y625
Applications
ELISA
Applications Notes
GPC-6 ELISA Kit allows for the in vitro quantitative determination of GPC-6 concentrations in serum, plasma, tissue homogenates and other biological fluids.