Human gonadotropin-releasing hormone,GnRH ELISA Kit

Human gonadotropin-releasing hormone,GnRH ELISA Kit

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Mfr.No.

CSB-E06880h

Sensitivity

0.625 pg/mL

Detection Range

2.5 pg/mL- 100 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

gonadotropin-releasing hormone 1 (luteinizing-releasing hormone), GNRH, GRH, LHRH, LNRH, gonadotropin-releasing hormone 1, gonadotropin-releasing hormone 1 (leutinizing-releasing hormone), gonadotropin-releasing hormone 1 (lutienizing hormone-releasing hormone)

Recovery

The recovery of mouse DKK3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	93	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse DKK3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	101
	Range %	97-104
1:4	Average %	83
	Range %	80-86
1:8	Average %	104
	Range %	100-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	2.289	2.487	2.388	2.204
300	1.582	1.532	1.557	1.373
150	0.881	0.898	0.890	0.706
75	0.530	0.502	0.516	0.332
37.5	0.382	0.375	0.379	0.195
18.8	0.312	0.306	0.309	0.125
9.4	0.251	0.248	0.250	0.066
0	0.182	0.186	0.184

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01148

Applications	ELISA
Research Area	GNRH1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.