Human golgi protein 73,GP-73 ELISA Kit

Human golgi protein 73,GP-73 ELISA Kit

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Mfr.No.

CSB-E11332h

Sensitivity

0.039 IU/mL

Detection Range

0.156 IU/mL-10 IU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

golgi membrane protein 1, RP11-379P1.3, C9orf155, FLJ22634, FLJ23608, GOLPH2, GP73, PSEC0257, bA379P1.3, golgi membrane protein GP73, golgi phosphoprotein 2, golgi protein, 73-kD

Recovery

The recovery of rat MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	95	92-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	99
	Range %	95-103
1:4	Average %	92
	Range %	88-96
1:8	Average %	95
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.333	2.173	2.253	2.086
10	1.402	1.377	1.390	1.223
5	0.868	0.888	0.878	0.711
2.5	0.598	0.583	0.591	0.424
1.25	0.425	0.412	0.419	0.252
0.625	0.298	0.312	0.305	0.138
0.312	0.260	0.251	0.256	0.089
0	0.168	0.166	0.167	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8NBJ4

Applications	ELISA
Research Area	GOLM1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.