Human Glypican-3,GPC-3 ELISA Kit

Human Glypican-3,GPC-3 ELISA Kit

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Mfr.No.

CSB-E11333h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glypican 3, AC002420.1, DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican proteoglycan 3, heparan sulphate proteoglycan, intestinal protein OCI-5, secreted glypican-3

Recovery

The recovery of human MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-100
EDTA plasma (n=4)	98	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	93
	Range %	85-101
1:4	Average %	96
	Range %	90-102
1:8	Average %	99
	Range %	90-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.608	2.600	2.604	2.474
50	1.765	1.696	1.731	1.601
25	1.153	1.144	1.149	1.019
12.5	0.687	0.667	0.677	0.547
6.25	0.421	0.397	0.409	0.279
3.12	0.258	0.247	0.253	0.123
1.56	0.187	0.183	0.185	0.055
0	0.132	0.128	0.130	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P51654

Applications	ELISA
Research Area	GPC3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502564	CSB-E11333h	96T	$695	Add to Cart
BHE10502564	CSB-E11333h	5x96T	$3336	Add to Cart
BHE10502564	CSB-E11333h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.