Human glypican 1 (GPC1) ELISA kit

Mfr.No.

CSB-EL009703HU

Sensitivity

0.039 ng/ml

Detection Range

0.156 ng/ml-10 ng/ml

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	107
	Range %	103-110
1:8	Average %	110
	Range %	104-114

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P35052

Applications	ELISA
Research Area	GPC1

Storage	Store at 2-8°C. Please refer to protocol.

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