Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH/G3PDH) ELISA kit

Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH/G3PDH) ELISA kit

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Mfr.No.

CSB-E13911h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

Recovery

The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	95	92-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	100
	Range %	97-104
1:4	Average %	90
	Range %	87-94
1:8	Average %	100
	Range %	95-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.260	2.232	2.246	2.078
20	1.454	1.486	1.470	1.302
10	0.920	0.928	0.924	0.756
5	0.654	0.663	0.659	0.491
2.5	0.403	0.398	0.401	0.233
1.25	0.322	0.314	0.318	0.150
0.625	0.266	0.257	0.262	0.094
0	0.170	0.166	0.168	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04406

Applications	ELISA
Research Area	GAPDH, G3PDH

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502549	CSB-E13911h	96T	$790	Add to Cart
BHE10502549	CSB-E13911h	5x96T	$3792	Add to Cart
BHE10502549	CSB-E13911h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.