Human Glycated hemoglobin A1c,GHbA1c ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08139h

Sensitivity

0.012 ng/mL

Detection Range

0.012 ng/mL-50 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Glycated hemoglobin A1c,GHbA1c

Recovery

The recovery of rat DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-97
EDTA plasma (n=4)	97	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	87
	Range %	81-92
1:200	Average %	96
	Range %	90-100
1:400	Average %	95
	Range %	91-104
1:800	Average %	92
	Range %	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	GHbA1c

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Detection of Total and A1c-Glycosylated Hemoglobin in Human Whole Blood Using Sandwich Immunoassays on Polydimethylsiloxane-Based Antibody Microarrays Chen HH et al,Anal Chem,2012 Oct 16;84(20):8635-41. Fabrication of Biosensor for Selective Electrochemical Determination of Glycated Hemoglobin Min Li.et al,Int. J. Electrochem. Sci,Aug 13

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