Human Glutaredoxin-related Protein 5 - Mitochondrial

Mfr.No.

E2717Hu

Sensitivity

7.08ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GLRX 5;GLRX5;Glutaredoxin-related protein 5, mitochondrial;Monothiol glutaredoxin-5

Molecular Weight

16628

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2717Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	974	31.71	3.3
Intra-Assay	2	18	359.2	21.88	6.1
Intra-Assay	3	18	571.7	28.52	5

Background

Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed: 20364084, PubMed: 23615440). Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed: 20364084, PubMed: 23615440, PubMed: 24334290). Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed: 20364084).

Protocol

These standard curves of E2717Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.488	2.558	2.52
	2.628
800ng/L	1.473	1.446	1.408
	1.419
400ng/L	0.772	0.747	0.709
	0.722
200ng/L	0.519	0.494	0.457
	0.47
100ng/L	0.198	0.209	0.172
	0.221
0ng/L	0.039	0.038	0
	0.037

Gene ID	51218
UniProt ID	Q86SX6

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104255	E2717Hu	48T	$320		Add to Cart
BHE12104255	E2717Hu	96T	$458		Add to Cart

Human Glutaredoxin-related Protein 5 - Mitochondrial - GLRX5 ELISA Kit

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