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Home / ELISA Kit

Human Glutamine-rich protein 2 (QRICH2) ELISA Kit

SKU : BHE13703215

RICH2 contains 1 CARD domain. Glutamine is one of the 20 amino acids encoded by the standard genetic code. It is not an essential amino acid. Its side-chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. Therefore, it can be considered the amide of glutamic acid.
Caspase activation and recruitment domains (CARDs), are interaction motifs found in a wide array of proteins, typically those involved in processes relating to inflammation and apoptosis. These domains mediate the formation of larger protein complexes via direct interactions between individual CARDs. CARD domains are found on a strikingly wide range of proteins, including helicases, kinases, mitochondrial proteins, caspases, and other cytoplasmic factors.

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Mfr.No.	KTE60999
Description	This Human Glutamine-rich protein 2 (QRICH2) ELISA Kit employs a two-site sandwich ELISA to quantitate QRICH2.
Alternative Names	QRICH2; DKFZp434P0316;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Glutamine-rich protein 2 microplate Human Glutamine-rich protein 2 standard Human Glutamine-rich protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Glutamine-rich protein 2 (QRICH2) ELISA Kit has high sensitivity and excellent specificity for detection of Human QRICH2. No significant cross-reactivity or interference between Human QRICH2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RICH2 contains 1 CARD domain. Glutamine is one of the 20 amino acids encoded by the standard genetic code. It is not an essential amino acid. Its side-chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. Therefore, it can be considered the amide of glutamic acid. Caspase activation and recruitment domains (CARDs), are interaction motifs found in a wide array of proteins, typically those involved in processes relating to inflammation and apoptosis. These domains mediate the formation of larger protein complexes via direct interactions between individual CARDs. CARD domains are found on a strikingly wide range of proteins, including helicases, kinases, mitochondrial proteins, caspases, and other cytoplasmic factors.
Notes	This Human Glutamine-rich protein 2 (QRICH2) ELISA Kit employs a two-site sandwich ELISA to quantitate QRICH2 in samples. An antibody specific for QRICH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRICH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRICH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRICH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	84074
Antigen	QRICH2
Tag	QRICH2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Glutamine-rich protein 2 (QRICH2) ELISA Kit employs a two-site sandwich ELISA to quantitate QRICH2 in samples. An antibody specific for QRICH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRICH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRICH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRICH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703215	KTE60999	48 T	$339		Add to Cart
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