Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2) ELISA kit

Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2) ELISA kit

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Mfr.No.

CSB-E17834h

Sensitivity

0.039 mU/mL

Detection Range

0.156 mU/mL-10 mU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2), FLJ40994, KAT4, KATIV, mitAAT, aspartate aminotransferase 2, kynurenine aminotransferase IV

Recovery

The recovery of mouse DLK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	89	82-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse DLK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-96
1:2	Average %	95
	Range %	91-99
1:4	Average %	94
	Range %	90-98
1:8	Average %	102
	Range %	92-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.045	1.944	1.995	1.844
500	1.644	1.523	1.584	1.433
250	1.073	1.052	1.063	0.912
125	0.673	0.662	0.668	0.517
62.5	0.371	0.382	0.377	0.226
31.2	0.284	0.273	0.279	0.128
15.6	0.235	0.231	0.233	0.082
0	0.148	0.153	0.151	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

UniProt ID	P00505

Applications	ELISA
Research Area	GOT2

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502531	CSB-E17834h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.