Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit

Mfr.No.

CSB-E15971h

Alternative Names

glutamic acid decarboxylase 65(GAD65) antibody (IgG)

Reactivity

Human

Detection Range

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Sample Type

Serum

Assay Type

Indirect

Assay Duration

1-5h

Recovery

The recovery of human GRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	105	101-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	81-86
1:2000	Average %	85
	Range %	82-89
1:4000	Average %	104
	Range %	101-107
1:8000	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	1.943	2.105	2.024	1.942
250	1.566	1.530	1.548	1.466
125	1.019	1.111	1.065	0.983
62.5	0.691	0.705	0.698	0.616
31.2	0.410	0.431	0.421	0.339
15.6	0.256	0.241	0.249	0.167
7.8	0.178	0.168	0.173	0.091
0	0.081	0.083	0.082	?

Species

Homo sapiens

Sensitivity

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Limit Of Detection

450 nm

Antigen	glutamic acid decarboxylase 65(GAD65) antibody (IgG)

Applications

ELISA

Research Area

IgG

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immune response to extracellular vesicles from human islets of Langerhans in patients with Type 1 diabetes Rutman AK.et al,Endocrinology,Octobert 10

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SKU	Supplier No.	Size	Your price
BHE10502527	CSB-E15971h	96T	$695	Add to Cart
BHE10502527	CSB-E15971h	5x96T	$3336	Add to Cart
BHE10502527	CSB-E15971h	10x96T	$6602.50	Add to Cart

Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit

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