Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit

Mfr.No.

CSB-E15971h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

glutamic acid decarboxylase 65(GAD65) antibody (IgG)

Recovery

The recovery of human GRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	105	101-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	81-86
1:2000	Average %	85
	Range %	82-89
1:4000	Average %	104
	Range %	101-107
1:8000	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Immune response to extracellular vesicles from human islets of Langerhans in patients with Type 1 diabetes Rutman AK.et al,Endocrinology,Octobert 10

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